Journal: Frontiers in Pharmacology

Article Title: The transmembrane channel-like 6 (TMC6) in primary sensory neurons involving thermal sensation via modulating M channels

doi: 10.3389/fphar.2024.1330167

Figure Lengend Snippet: TMC6 in DRG modulates heat sensitivity of mice. (A) Schematic diagram depicts the design of viruses. TMC6 knock-down with AAV9-mTMC6-shRNA in male WT mice; TMC6 gene overexpression with AAV9-mTMC6-cDNA in TMC6-KO male mice and their respective control. (B) Schematic diagram depicts the sites (L4 DRG) for the injections of designed viruses in mice. (C) Confirmation of EGFP (green) expression in L4 DRG of the WT male mice received AAV9-mTMC6-shRNA virus and TMC6-KO male mice received AAV9-mTMC6-cDNA virus viral infection. Scale bars, 50 μm. (D–G) The Hargreaves test (D,F) and Von Frey test (E,G) were performed after injection the AAV9-mTMC6-shRNA and AAV9-mTMC6-cDNA virus. (H–K) The comparison of chronic inflammatory pain induced by CFA injection between TMC6-KO male mice and their WT littermates. Hargreaves (H,J) and Von Frey tests (I,K) were used to assay the chronic pain behaviours. Data are shown as mean ± SEM. two-way repeated ANOVA with the Tukey post-test was used for comparison of behavioural measurements within the corresponding time points: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Knockdown mTMC6 gene of WT mice and re-express TMC6 of TMC6-KO mice in the L4 DRG were achieved by affecting the DRG with AAV9 virus, and were produced from Genechem Co., Ltd (Shanghai, China).

Techniques: Knockdown, shRNA, Over Expression, Control, Expressing, Virus, Infection, Injection, Comparison